SOCS1 Regulates Apoptosis and Inflammation by Inhibiting IL-4 Signaling in IL-1β-Stimulated Human Osteoarthritic Chondrocytes

Figure 3

Knockdown of SOCS1 suppressed the production of inflammatory factors in IL-1β-stimulated human osteoarthritic chondrocytes transfected with and without siIL-4. Inflammatory factors, including IL-6 (a), TNF-alpha (b), iNOS (d), and IFN-gamma (e), were measured by RT-PCR. Protein production of TNF-alpha was detected in culture supernatants by ELISA (c). IL-1β: 10 ng/ml IL-1β stimulation for 24 h (); siSOCS1: transfection with SOCS1 siRNA (); siIL-4: transfection with IL-4 siRNA (); “−”: no added, “+”: added. versus no addition, versus no addition, versus only added IL-1β, versus only added IL-1β, .

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