SOCS1 Regulates Apoptosis and Inflammation by Inhibiting IL-4 Signaling in IL-1β-Stimulated Human Osteoarthritic Chondrocytes

Figure 4

Knockdown of SOCS1 upregulated IL-1β-induced IL-4 expression in human osteoarthritic chondrocytes. Expression of IL-4 at the protein (a) and mRNA (b) levels was detected using Western blotting and RT-PCR, respectively. Additionally, the concentration of IL-4 was measured by ELISA (c). Ctrl: control (); IL-1β: human osteoarthritic chondrocytes were stimulated with 10 ng/mL IL-1β for 24 h (); IL-1β + siCtrl: human osteoarthritic chondrocytes were transfected with the negative control after IL-1β stimulation (); IL-1β + siSOCS1: human osteoarthritic chondrocytes were transfected with SOCS1 siRNA after IL-1β stimulation (). versus Ctrl, versus IL-1β, versus IL-1β, , .

(a)

(b)

(c)