SOCS1 Regulates Apoptosis and Inflammation by Inhibiting IL-4 Signaling in IL-1β-Stimulated Human Osteoarthritic Chondrocytes

Table 1

Primer sequences.

	Forward (5′-3′)	Reverse (5′-3′)	Length of production (bp)	Efficiency (%)	Tm (°C)	Accession number
SOCS1	CTTCCTCCTCTTCCTCCTC	GCCATCTTCACGCTAAGG	286	93.7	50.8	NM_003745.1
IL-6	GTGAGGAACAAGCCAGAG	TGACCAGAAGAAGGAATGC	283	101.2	50.1	NM_000600.4
TNF-alpha	TCCAGACTTCCTTGAGACA	GGCGATTACAGACACAACT	369	104.8	50.7	NM_000594.3
iNOS	CTCAGCAAGCAGCAGAAT	CGTCAGTTGGTAGGTTCC	402	103.5	49.8	L24553.1
IFN-gamma	GGTTCTCTTGGCTGTTACT	ATGTCTTCCTTGATGGTCTC	251	98.5	50.1	NM_000619.2
IL-4	CCTCTGTTCTTCCTGCTAG	CTCTGGTTGGCTTCCTTC	365	99.3	51.6	NM_000589.3
GAPDH	GGCTCTCCAGAACATCATC	TCTTCCTCTTGTGCTCTTG	448	103.9	50.5	NM_001256799.2