Protective Effects of the Segmental Renal Artery Clamping Technique on Ischemia-Reperfusion Injury in db/db Diabetic Mice

Figure 5

SRAC protects mitochondrial structure during renal ischemia-reperfusion. Kidney sections obtained 24 h after surgery were examined by electron microscopy (×80,000). Mitochondria in sham group mice are elongated with densely packed cristae membranes (a, e). Mitochondria in nonischemic area of SRAC group are also elongated with numerous cristae membranes, although cristae are not as densely packed as in sham group (d, h). Mitochondria from RAC group mice are grossly swollen and cristae are collapsed together with an extremely reduction of matrix density (b, f). Although most mitochondria are still rounded in ischemic area of SRAC group, they contain more cristae membranes and have more condensed matrix density than RAC group (c, g). The overall morphological structure changing of mitochondria in GSE(+) and GSE(−) group is similar besides the matrix density.