Production of Human Cu,Zn SOD with Higher Activity and Lower Toxicity in E. coli via Mutation of Free Cysteine Residues

Figure 4

Toxicity of wtSOD1 and mhSOD1/C111S to BHK cells. BHK cells were treated with recombinant SOD1s, which were serially diluted with RPMI 1640 medium, for 1–5 days and cell proliferation was assessed by CCK-8 method. BSA was used as the negative control. compared between indicated groups. Data are representative of three experiments.