Isolation of Fibroblast-Activation Protein-Specific Cancer-Associated Fibroblasts

Figure 2

Cancer-associated fibroblasts (CAFs) colonies from tumor before purification by magnetic beads. (a) Phase contrast images and α-SMA expression of CAFs after isolation by different digestion procedures. Normal fibroblasts (NFs) as negative control cells isolated from normal human tissue (such as skin) (scale bars represent 100 μm and 50 μm). (b) Western blot analysis showed the FAP expression of adherent cells by different time duration of collagenase type IV digested with a vortex. (c) The statistical chart of the number of adherent cells after isolation by different digestion procedures. Data are presented as the mean ± SEM (). NS, not significant; .

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