Isolation of Fibroblast-Activation Protein-Specific Cancer-Associated Fibroblasts

Figure 5

FAP expression of CAFs after purification by magnetic beads. (a) Immunocytochemistry/immunofluorescence (ICC/IF) results of FAP⁺ CAFs (scale bars represent 50 μm). (b) Fluorescence-Activated Cell Sorting (FACS) showed the FAP expression of CAFs. (c) Western blot analysis showed the FAP expression of CAFs. Normal fibroblasts (NFs) as negative control cells isolated from normal human tissue (such as skin).

(a)

(b)

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