Peptide Fraction pOh2 Exerts Antiadipogenic Activity through Inhibition of C/EBP-α and PPAR-γ Expression in 3T3-L1 Adipocytes

Figure 2

Effect of PFs on adipogenesis in 3T3-L1 adipocytes. (a) Intracellular lipid was stained with Oil Red O on differentiated 3T3-L1 adipocytes treated with adipogenesis cocktail in the presence or absence of PFs (1, 2, 3, and 4) at 10 μg/mL for 8 days (from day 0 to day 8). (b) Quantitative analysis of Oil Red O staining. To determine the extent of adipose conversion, 1 mL Oil Red O solution was added to the 12-well plates. The extracted dye was removed after 15 minutes of incubation at RT and its optical density was monitored at 510 nm. (c) The viability of 3T3-L1 adipocytes. Results are the mean ± SD of three independent experiments conducted in triplicate. NS, not significant; as compared with differentiated 3T3-L1 adipocytes.

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