Physicochemical and Biological Characterization of the Proposed Biosimilar Tocilizumab

Figure 10

Comparison of inhibition of STAT3 phosphorylation between HS628 and originator tocilizumab (Actemra). The dose-response curves of inhibition effects on STAT3 phosphorylation of HS628 and originator tocilizumab (Actemra) were generated using 4-parameter-curve equation y = A1 + (A2 − A1)/(1 + ) by the software OriginPro (Version 8.6.0 Sr2). The EC50 values of STAT3 phosphorylation inhibition of HS628 and originator were calculated from the logx0 values from the corresponding curves. The results showed that the dose-response curves of HS628 and tocilizumab were highly overlapped, indicating that the inhibition effect on STAT3 phosphorylation of HS628 was highly comparable with that of originator tocilizumab (Actemra).