Physicochemical and Biological Characterization of the Proposed Biosimilar Tocilizumab

Table 2

The whole molecule, heavy chain, and light chain exact masses of originator tocilizumab and HS628 by MS.

Type		Originator tocilizumab (4 bathes); Mass (Da)				HS628 (4 bathes); Mass (Da)
		B2014B10	B2019B26	B1018B13	B2027B13	20130901	20130902	20130903	20130904
Whole; molecule	G0F/G0F	147885.19	147884.44	147885.81	147885.61	147885.78	147887.00	147882.39	147887.77
	G0F/G1F	148046.00	148045.88	148046.81	148046.61	148046.27	148048.14	148044.20	148048.41
	G1F/G1F	148207.06	148206.64	148207.81	148207.63	148208.34	148208.23	148204.53	148209.59
	G1F/G2F	148365.19	148364.81	148365.66	148365.53	148366.00	148369.11	148364.52	148371.30
	Deglycosylated molecule	144998.63	144996.91	144997.30	144997.67	144997.02	144997.81	144994.66	144999.08
Heavy; chain	G0F	50445.32	50445.18	50445.46	50445.50	50443.57	50443.88	50444.11	50443.75
	G1F	50606.94	50606.87	50606.97	50607.01	50605.44	50605.91	50605.71	50605.55
	G2F	50767.95	50767.80	50767.92	50767.96	50772.80	50772.06	50774.06	50776.70
	Deglycosylated molecule	48999.88	48999.73	48999.92	49000.03	48997.97	48998.05	48998.36	48998.13
Light chain		23500.97	23500.96	23500.97	23500.96	23500.75	23500.75	23500.77	23500.76