Serum Amyloid A Induces a Vascular Smooth Muscle Cell Phenotype Switch through the p38 MAPK Signaling Pathway

Figure 1

SAA induces RASMC dedifferentiation. The RASMCs were treated with SAA at indicated concentrations for 72 h. (a) mRNA expression of the smooth muscle cell (SMC) related gene (α-SMA, h1-calponin, and SM22α) and CRBP-1 was examined by qPCR analysis. (b) Expression of the smooth muscle cell (SMC) related markers (α-SMA, h1-calponin, and SM22α) and CRBP-1 was detected by Western blot analysis. (c) Statistical analysis of the Western blot results. (d) Expression of α-SMA, h1-calponin, and SM22α was detected by immunofluorescent staining. , versus the control group. Data shown are means ± SD from three independent experiments.

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