Serum Amyloid A Induces a Vascular Smooth Muscle Cell Phenotype Switch through the p38 MAPK Signaling Pathway

Figure 4

Inhibition of ERK1/2 does not affect the SAA mediated RASMC phenotype switch. RASMCs were pretreated with the ERK1/2 inhibitor U0126 (10 μM) for 30 min followed by SAA treatment. (a) Western blot analysis of phosphorylated and total ERK1/2 protein expression. (b) qPCR analysis of the effect of the ERK1/2 inhibitor on SAA-induced RASMC phenotype modulation. (c) Western blot analysis of the effect of ERK1/2 inhibitor on SAA-induced RASMC phenotype modulation and the phosphorylated and total p38. Data shown are means ± SD from three independent experiments.

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