Identification of IFITM1 and IFITM3 in Goose: Gene Structure, Expression Patterns, and Immune Reponses against Tembusu Virus Infection

Table 1

List of primers and sequences.

Primer name	Primer sequence (5′-3′)	Instruction
IFITM1-F	TCTA(C/G)CCCGCC(A/C)CCCAAG	Partial sequence
IFITM1-R	GGCTC(C/T)T(C/G)GCTGTCCTCC
IFITM3-F	CCACCTGGCTTGGTCGCT
IFITM3-R	GGCGATGATGAGGATGATG
AP	CCAGTGAGCAGAGTGACGAGGACTCGAGCTCAAGC(T)18	3RACE
AP1	CCAGTGAGCAGAGTGACG
AP2	GAGGACTCGAGCTCAAGC
AAP	GGCCACGCGTCGACTACGGGIIGGGIIGGGIIGGGIIG	5RACE
AUAP	GGCCACGCGTCGACTAGTAC
IFITM1-5′GSP0	TGGCTGTCCTCCCGT	5RACE-GSP
IFITM1-5′GSP1	GGTCCTTCGCCACTGTCCTATC
IFITM1-5′GSP2	CGAGGAAGCCCAGGCAGAAG
IFITM3-5′GSP0	GGCGATGATGAGGATG
IFITM3-5′GSP1	GGAAGCCGAGGCAGCAGAC(A/G)TTG
IFITM3-5′GSP2	GGAGCACAGCGACCAAGCCAG
IFITM1-3′GSP1	GTGCTCTGGTCTTTCTTCAACG	3RACE-GSP
IFITM1-3′GSP2	CGAGATAGGACAGTGGCGAAC
IFITM1-5′F	TGCTCCAACTCCCCGCTGCT	Complete coding sequence
IFITM1-3′R	CGTGGTGCTGCCTCTCCTC
IFITM3-5′F	AAGCAGGGATGGAGCGGAC
IFITM3-3′R	CGGGGAAGGGGCTA(T/A)GTG
IFITM1-qPCR-F	TCTGGTCTTTCTTCAACGCC	Real-time PCR
IFITM1-qPCR-R	TTGAGGCTCCTGGCTGTCC
IFITM3-qPCR-F	CCACCTGGCTTGGTCGCT
IFITM3-qPCR-R	CGCTGTAGTCGCCGAGGA
GAPDH-qPCR-F	CATCTTCCAGGAGCGCGACC
GAPDH-qPCR-R	AGACACCGGTGGACTCCACA