Coutilization of D-Glucose, D-Xylose, and L-Arabinose in Saccharomyces cerevisiae by Coexpressing the Metabolic Pathways and Evolutionary Engineering

Figure 2

The growth curves of strains BSW3AP (◆), BSW4XA1 (■), BSW4XA2 (▲), and BSW4XA3 (●) on 20 g L⁻¹ D-glucose (a), D-xylose (b), and L-arabinose (c), respectively. The strains were preincubated in SC-Leu-Ura medium containing 20 g L⁻¹ D-glucose for 24 h and then transferred using 10 g L⁻¹ D-glucose and 20 g L⁻¹ L-arabinose as the carbon sources to incubate for 24 h. The cells were then collected and used for aerobiotic batch cultivation in 40 mL SC-Leu-Ura medium with 20 g L⁻¹ D-glucose, D-xylose, or L-arabinose at 30°C, 200 r min⁻¹, and the initial OD₆₀₀ was 0.3. The data are the averages of three independent tests.

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