Coutilization of D-Glucose, D-Xylose, and L-Arabinose in Saccharomyces cerevisiae by Coexpressing the Metabolic Pathways and Evolutionary Engineering

Table 1

S. cerevisiae strains and plasmids used in this work.

	Genotype/properties	Source/reference
Strain
BSW3AP	CEN.PK102-3A derivative, gre3(−241, +338):: TPI1p–RKI1–RKI1t–PGK1p–TAL1–TAL1t–FBA1p–TKL1–TKL1t–ADH1p–RPE1–RPE1t–loxP, YIp5-ara, pYX2422-TEF1araA, selected for growth on L-arabinose	[29]
BSW4AP	BSW3AP derivative, discarding plasmid pYX2422-TEF1araA	Present work
BSW4XA1	BSW4AP derivative, pYX242-XIA	Present work
BSW4XA2	BSW4XA1 derivative, pYX242-XIA, pYMIK-xy127	Present work
BSW4XA3	BSW4XA2 derivative, selected for growth on D-xylose	Present work
Plasmid
pYX2422-TEF1araA	pYX242-PGK1t-TEF1p-araA	[29]
pYX242-XIA	pYX242-Ru-xylA-PGK1t-TEF1p-araA	Present work
pYMIK-xy127	Integration plasmid, KanMX4, ADH1p-XYL1-ADH1t, PGK1p-XYL2-PGK1t, PGK1p-XKS1-PGK1t	[32]