XRRA1 Targets ATM/CHK1/2-Mediated DNA Repair in Colorectal Cancer

Figure 3

Overexpression of XRRA1 induces cell proliferation in HT29 and HCT116 cell lines. (a) The infection efficiency of XRRA1 was observed by fluorescence microscopy. (b) MTT assay shows that overexpression of XRRA1 in HT29 and HCT116 cells increased cell growth. (c) The percentage of cells that incorporated EdU was determined by examining at least 200 cells per sample in multiple fields. Data in (d) are averages of three repeats. (e) Cell cycle analysis of XRRA1 overexpression in HT29 and HCT116 cells by flow cytometry. Data in (f) are averages of three repeats. (g) Western blot analyses of cyclin E, p21, and cyclin A in HT29 and HCT116 cells after XRRA1 lentivirus infection. (h) Quantitative real-time PCR analysis of cyclin E, p21, and cyclin A in HT29 and HCT116 cells after XRRA1 lentivirus infection.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)