miR-373-3p Targets DKK1 to Promote EMT-Induced Metastasis via the Wnt/-Catenin Pathway in Tongue Squamous Cell Carcinoma

Figure 2

miR-373-3p promotes TSCC cell EMT and invasion in vitro. (a) After SCC-9 and UM1 cells were transfected with miR-NC (50 nM), miR-373-3p (50 nM), and orantagomiR-373-3p (50 nM) for 24 h, the efficacy of miR-373-3p expression was determined by qRT-PCR. ((b) and (c)) Real-time PCR (b) and Western blotting (c) analysis revealed that miR-373-3p regulates the expression levels of numerous EMT regulators. The pseudocolors represent the log₂ transformed intensity scales of miR-373-3p expression versus control-transfected cells or antagomiR-373-3p versus control-transfected cells. (d) Proliferation curves showing the effect of miR-373-3p on cell proliferation. ((e) and (f)) Representative micrographs and histograms depicting the invasion of SCC-9 and UM1 cells after miR-NC (50 nM), miR-373-3p (50 nM), or antagomiR-373-3p (50 nM) transfection. The data are presented as the mean ± s.d. of three independent experiments. , and .

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