Identification and Characterization of Oleaginous Yeast Isolated from Kefir and Its Ability to Accumulate Intracellular Fats in Deproteinated Potato Wastewater with Different Carbon Sources

Table 1

Results of an identification of kefir yeast isolates using API 20 AUX and genetic analysis.

API name isolate identification	Origin	bp	Name of isolate; GenBank number
Candida; inconspicua/; Candida; krusei; 99.5%	1 caaaaaacaa aactttcaac aacggatctc ttggttctcg catcgatgaa gagcgcagcg; 61 aaatgcgata cctagtgtga attgcagcca tcgtgaatca tcgagttctt gaacgcacat; 121 tgcgccctct ggtattccgg agggcatgcc tgtttgagcg tcgtttcctt cttgcttgcg; 181 agcagaaatg ggggggccct ggcattgggg ccgctctgaa aagaaacgtt gcgggcgaag; 241 cgaactatga gtaggacgct tggccgccga acttaataca taagctcgac ctcaaatcag; 301 gtaggaatac ccgctgaact taagca	326	Candida inconspicua IG 11; LC164191.1
Candida; guilliermondii 1; 99.1%	1 acttttgctt tggtctggac tagaaatagt ttgggccaga ggtttactga actaaacttc; 61 aatatttata ttgaattgtt atttatttaa ttgtcaattt gttgattaaa ttcaaaaaat; 121 cttcaaaact ttcaacaacg gatctcttgg ttctcgcatc gatgaagaac gcagcgaaat; 181 gcgataagta atatgaattg cagattttcg tgaatcatcg aatctttgaa cgcacattgc; 241 gccctctggt attccagagg gcatgcctgt ttgagcgtca tttctctctc aaaccttcgg; 301 gtttggtatt gagtgatact cttagttgaa ctaggcgttt gcttgaaatg tattggcatg; 361 agtggtactg gatagtgcta tatgactttc aatg	394	Debaryomyces hansenii 1; LC126697.1
Candida; famata; 99.9%	1 gaacttttgc tttggtctgg actagaaata gtttgggcca gaggtttact gaactaaact; 61 tcaatattta tattgaattg ttatttattt aattgtcaat ttgttgatta aattcaaaaa; 121 atcttcaaaa ctttcaacaa cggatctctt ggttctcgca tcgatgaaga acgcagcgaa; 181 atgcgataag taatatgaat tgcagatttt cgtgaatcat cgaatctttg aacgcacatt; 241 gcgccctctg gtattccaga gggcatgcct gtttgagcgt catttctctc tcaaaccttc; 301 gggtttggta ttgagtgata ctcttagttg aactaggcgt ttgcttgaaa tgtattggca; 361 tgagtggtac tggatagtgc tatatgactt tcaatgtatt aggtttatcc aactcgttga; 421 atagtttaat ggtatatttc tcg	443	Debaryomyces hansenii IG 01; LC164190.1
Candida; guilliermondii 2; 99.1%	1 cttttgcttt ggtctggact agaaatagtt tgggccagag gtttactgaa ctaaacttca; 61 atatttatat tgaattgtta tttatttaat tgtcaatttg ttgattaaat tcaaaaaatc; 121 ttcaaaactt tcaacaacgg atctcttggt tctcgcatcg atgaagaacg cagcgaaatg; 181 cgataagtaa tatgaattgc agattttcgt gaatcatcga atctttgaac gcacattgcg; 241 ccctctggta ttccagaggg catgcctgtt tgagcgtcat ttctctctca aaccttcggg; 301 tttggtattg agtgatactc ttagttgaac taggcgtttg cttgaaatgt attggcatga; 361 gtggtactgg atagtgctat atgactttca atgtattagg tttatccaac tcgttgaata; 421 gtttaa	426	Debaryomyces hansenii IG II; LC164187.1
Saccharomyces cerevisiae; 61.0%	1 gccgaaccag cgcttaattg cgcggtttgg tgggtctctg tagctcagta gcactattac; 61 acactgtgga gattttataa ttctttgcat gcttctttgg gcagcttcgg cagcccagag; 121 gtaacaaaca caaacaactt tgtaatattt ttaacccagt caaaaccaga attccagaaa; 181 gatttatctt tttgtaatat tataacaaat attcaaaact ttcaacaacg gatctcttgg; 241 ttctcgcatc gatgaagaac gcagcgaaat gcgatacgta atgtgaattg cagaattccg; 301 tgaatcatcg aatctttgaa cgcacattgc gccccttg	338	Kazachstania unispora IG 16; LC164188.1
Candida kefyr; 99.9%	1 ttctcatcct aaacacaatg gagttttttc tctatgaact acttccctgg agagctcgtc; 61 tctccagtgg acataaacac aaacaatatt ttgtattatg aaaaactatt atactataaa; 121 atttaatatt caaaactttc aacaacggat ctcttggttc tcgcatcgat gaagaacgca; 181 gcgaattgcg atatgtattg tgaattgcag attttcgtga atcatcaaat ctttgaacgc; 241 acattgcgcc ctctggtatt ccagggggca tgcctgtttg agcgtcattt ctctctcaaa; 301 cctttgggtt tggtagtgag tgatactcgt ctcgggttaa cttgaaagtg gctagccgtt; 361 gccatctgcg tgagcagggc tgcgtgtcaa gtctatggac t	401	Kluyveromyces marxianus IG 1; LC126698.1
Cryptococcus; albidus; 99.1%	1 ctgacacata cacacagtgg agatatattc tttcttcttc ttcttctttg ggggacggcg; 61 gtttgcgctg ctgctcccag aatgaaaaaa cacaaacaac ttttgtatta ttacagatgt; 121 caaaacacaa aacaaaatac caaaactttc aacaacggat ctcttggttc tcgcatcgat; 181 gaagaacgca gcgaaatgcg atacgtaatg tgaattgcag aattccgtga atcatcgaat; 241 ctttgaacgc acattgcgcc ccttggtatt ccagggggca tgcctgtttg agcgtcattt; 301 ccttctcaaa cacttgtgtt tggtagtgag tgatactctg taacatggga gttagcttga; 361 aattgagagc ccatgggctg ctctgctgag gcggaagtcg tgctaggtaa caccaactcg; 421 acgcaacgca ttcggctgga cgcagctccg gcgaacaaac acatcaacgc ttgacctcaa; 481 atca	484	Zygotorulaspora florentina IG 12

API: Api 20 AUX tests; bp: base pairs.