Comparative Phenotype and Genome Analysis of Cellvibrio sp. PR1, a Xylanolytic and Agarolytic Bacterium from the Pearl River
Table 1
Phenotype and fatty acid characteristics of Cellvibrio species.
1
2
3
4
5
6
16S rDNA similarity to PR1 (%)
100
98.6
98
97.4
97.3
95.7
G+C content (mol%)
49.98
48
47.4–48.4
52.6
44.6
44.9
Nitrate reduced to nitrite
+
+
+
−
+
+
Utilization of
Maltose
+
+
+
+
+
+
Arabinose
+
+
+
+
+
+
Mannose
−
+
+
+
−
−
N-Acetyl-D-glucosamine
+
+
−
+
+
+
Carboxymethyl cellulose
−
+
+
+
+
+
Agar
+
−
−
−
−
−
Chitin
+
−
−
−
+
+
Valine arylamidase
−
−
+
−
−
−
Acid phosphatase
+
+
+
−
−
−
β-Galactosidase
+
−
−
−
−
−
β-Glucosidase
+
+
+
+
+
−
α-Galactosidase
+
+
+
−
+
+
β-Glucuronidase
+
−
−
−
+
+
α-Fucosidase
+
−
−
−
−
−
Leucine arylamidase
−
+
+
+
+
+
Naphthol-AS-BI-phosphohydrolase
−
−
+
+
+
+
Growth at 4°C (14 days)
−
+
+
−
+
−
Growth at 37°C
+
−
−
−
−
+
Mucoid growth on TSA
−
−
−
+
−
+
Yellow pigment on TSA
+
−
+
−
−
+
Fatty acid:
10:0
2.33
4.8–7.1
4.1–8.2
2.6–5.8
4.20
4.3–6.9
10:0 3-OH
2.57
7.7–10.7
5.5–13.4
13.00
6.50
6.1–10.9
12:0
1.99
5.4–7.7
4.0–7.2
tr
4.40
4.4–5.8
11:0 3-OH
—
0.0–1.1
tr
tr
—
—
12:0 2-OH
—
3.3–5.4
2.8–6.0
7.8–11.0
3.60
2.6–3.9
12:1 3-OH
4.06
—
—
—
1.30
—
12:0 3-OH
—
1.0–1.5
tr
tr
—
—
14:0
1.97
1.6–2.5
1.2–2.4
1.3–1.7
tr
0.9–1.2
15:0
—
0.0–1.3
tr
0.9–1.9
2.20
tr
16:0
36.74
15.7–20.0
18.5–27.8
14.3–18.0
19.70
17.6–20.9
17:0
2.40
1.6–4.5
0.0–1.9
2.2–3.7
5.70
1.3–2.0
17:1ω8c
—
tr
—
tr
tr
tr
18:0
8.78
0.0–2.8
0.0–1.6
tr
3.10
tr
18:1ω6c
—
—
0.0–2.8
0.0–3.3
—
0.0–4.3
18:1ω7c
—
11.4–19.0
7.3–15.3
11.3–17.9
16.80
12.9–19.8
18:1ω9c
2.87
—
—
—
—
—
19:0
2.90
—
—
—
—
—
20:0
6.81
—
—
—
—
—
ECL 11.799
—
—
—
—
—
tr
ECL 18.814
—
tr
—
—
—
—
Summed feature 2
2.47
—
—
—
—
—
Summed feature 3
17.37
25.6–34.0
34.4–38.1
30.4–34.9
30.90
34.1–38.2
Summed feature 8
6.75
—
—
—
—
—
Strains: 1, PR1; 2, Cellvibrio fibrivorans LMG 18561T; 3, Cellvibrio ostraviensis LMG 19434T; 4, Cellvibrio mixtus ACM 2601T; 5, Cellvibrio fulvus LMG 2847T; 6, Cellvibrio vulgaris NCIMB 8633T. —, not detected; tr, trace (≤1.0% of total). Unknown fatty acids are designated by their equivalent chain-length (ECL), relative to the chain lengths of known saturated fatty acids. Summed feature 2 comprises iso- I, 3-OH, and/or an unknown fatty acid. Summed feature 3 comprises iso 2-OH, ω7c, or both. Summed feature 8 comprises ω7c and/or ω6c. Data for related Cellvibrio species came from Humphry et al. [22] and Mergaert et al. [23].
