Research Article
Recombinant Lactococcus lactis Expressing Haemagglutinin from a Polish Avian H5N1 Isolate and Its Immunological Effect in Preliminary Animal Trials
Figure 1
Immunoblot detection of H5 production under nisA promoter in L. lactis NZ9000 cells at different induction times. M: marker, Precision Plus Protein Dual Color Standards (Bio-Rad). Protein extracts: lane 1, NZ[pNZ8150] (negative control); lanes 2, 3, and 4, NZ-H5 after nisin (5 ng ml−1) induction for 1, 2, or 3 hrs, respectively. Qinghai-HA(H5N1)(A/Bar-headed Goose/Qinghai/12/05) protein (Immune Technology) was used as a positive control (C+). The migration level of bands corresponding to detected H5 recombinant protein (63 kDa) is marked by a black arrow.