NOTCH1 Mutations in Aortic Stenosis: Association with Osteoprotegerin/RANK/RANKL

Figure 2

Structure of NOTCH1 EGF CA domain. (a) The sequence alignment of human NOTCH1 EGF CA domain with rat NOTCH1 EGF CA domain of known crystal structure (PDB ID: 4XLW Chain A) visualized in Jalview 2.8.2 [11]. Highly conserved residues (conservation score = 11) are marked as “” and identical residues (conservation score = 10) are marked as “+.” The amino acids in red boxes are mutated positions. (b) Three-dimensional structure of EGF modules of the NOTCH extracellular domain bound to DLL4 [12] visualized by PyMol. Structures of proteins are shown in cartoon representation. Calcium ions are represented as gray spheres. β-D-glucose (BGC) and -L-fucose (FUC) molecules are highlighted in yellow. Residues of interest E1305, D1267, and R1279 are marked as sticks in red color.

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