Evaluation of the Bacterial Diversity in the Human Tongue Coating Based on Genus-Specific Primers for 16S rRNA Sequencing

Table 2

The designed multigroup PCR primers.

Primer ID	Genus name	Primer sequence (5′ - 3′)	(°C)	Amplicon length (bp)
ActF	Actinomyces	P-E-GCGAAGAACCTTACCAAGGC	56.2	142
ActR	Actinomyces	Q-E-TGACGACAACCATGCACCAC
PreF	Prevotella	P-E-GAACCTTACCCGGGCTTGAA	54.2	138
PreR	Prevotella	Q-E-TGACGACAACCATGCAGCAC
StrF	Streptococcus	P-E-AACGATAGCTAATACCGCAT	46.1	139
StrR	Streptococcus	Q-E-TAATACAACGCAGGTCCATC
TreF	Treponema	P-E-CGCGAGGAACCTTACCTGGG	53.7	137
TreR	Treponema	Q-E-ACGACAGCCATGCAGCACCT
LepF	Leptotrichia	P-E-ACGCGAGGAACCTTACCAGA	53.5	139
LepR	Leptotrichia	Q-E-CAGCCATGCACCACCTGTCT
NeiF	Neisseria	P-E-CGGGTTGTAAAGGACTTTTG	49.2	135
NeiR	Neisseria	Q-E-AGTTAGCCGGTGCTTATTCT
SelF	Selenomonas	P-E-CTTCGGATCGTAAAGCTCTG	51.4	142
SelR	Selenomonas	Q-E-TAGTTAGCCGTGGCTTCCTC
CapF	Capnocytophaga	P-E-TACGCGAGGAACCTTACCAA	51.3	138
CapR	Capnocytophaga	Q-E-ACAACCATGCAGCACCTTGA
PorF	Porphyromonas	P-E-CAGCCAAGTCGCGTGAAGGA	54.0	172
PorR	Porphyromonas	Q-E-CTGGCACGGAGTTAGCCGAT
FusF	Fusobacterium	P-E-ACGCGTAAAGAACTTGCCTC	51.4	75
FusR	Fusobacterium	Q-E-ACGCGTAAAGAACTTGCCTC
MycF	Mycoplasma	P-E-GATGGAGCGACACAGCGTGC	55.2	187
MycR	Mycoplasma	Q-E-GCGGCTGCTGGCACATAGTT
DiaF	Dialister	P-E-CGGAATTATTGGGCGTAAAG	53.1	161
DiaR	Dialister	Q-E-CTTTCCTCTCCGATACTCCA
EubF	Eubacterium	P-E-GATACCCTGGTAGTCCACGC	51.6	146
EubR	Eubacterium	Q-E-CTCCCCAGGTGGAATACTTA
FirF	Firmicutes	P-E-GATACCCTGGTAGTCCACGC	51.6	146
FirR	Firmicutes	Q-E-CTCCCCAGGTGGAATACTTA
PepsF	Peptostreptococcus	P-E-TAATTCGAAGCAACGCGAAG	52.7	154
PepsR	Peptostreptococcus	Q-E-CGACAACCATGCACCACCTG
KinF	Kingella	P-E-GGAATTACTGGGCGTAAAGC	52.4	168
KinR	Kingella	Q-E-AATTCTACCCCCCTCTGACA
PepnF	Peptoniphilus	P-E-ATCACTGGGCGTAAAGGGTT	51.1	186
PepnR	Peptoniphilus	Q-E-CGCATTTCACCGCTACACTA
LachF	Lachnospiraceae	P-E-CAACGCGAAGAACCTTACCA	54.2	146
LachR	Lachnospiraceae	Q-E-GACGACAACCATGCACCACC
PasF	Aggregatibacter	P-E-CGGGTTGTAAAGTTCTTTCG	48.4	135
PasR	Aggregatibacter	Q-E-TTAGCCGGTGCTTCTTCTGT
PasF	Haemophilus	P-E-CGGGTTGTAAAGTTCTTTCG	48.4	135
PasR	Haemophilus	Q-E-TTAGCCGGTGCTTCTTCTGT
LactF	Lactobacillus	P-E-CCGCAACGAGCGCAACCCTT	55.3	103
LactR	Lactobacillus	CCTCCGGTTTGTCACCGGCA
TM7F	TM7	P-E-GGGCGTAAAGAGTTGCGTAG	49.2	185
TM7R	TM7	Q-E-TACGGATTTCACTCCTACAC
GemF	Gemella	AAAGCTCTGTTGTTAGGGAA	46.8	98
GemR	Gemella	P-E-GGTGGCTTTCTGGTTAGGTA
PseF	Pseudomonas	GGCGGCAGGCCTAACACATG	56.5	99
PseR	Pseudomonas	P-E-TTACTCACCCGTCCGCCGCT
VeiF	Veillonella	P-E-GTAAAGCTCTGTTAATCGGG	48.1	100
VeiR	Veillonella	GTGGCTTTCTATTCCGGTAC
MogF	Mogibacterium	P-E-CACGTGCTACAATGGTCGGT	50.7	101
MogR	Mogibacterium	ATCCGAACTGGGATCGGTTT
CatF	Catonella	P-E-CATGCAAGTCGAACGGAGAT	50.8	101
CatR	Catonella	GTTACTCACCCGTCCGCCAC

CCAAGGCGGCCGTACG; CTGAAG; CCGACGTCGACTATCCAT.