Plant Proteinase Inhibitor BbCI Modulates Lung Inflammatory Responses and Mechanic and Remodeling Alterations Induced by Elastase in Mice

Table 1

Effect of BbCI treatment on positive cells in alveolar septum of all groups. Data are presented as mean ± SE. ELA group had greater values than control groups (SAL and SALBC) in all alveolar septa parameters. The group that was treated with BbCI inhibitor (ELABC) had attenuated this response in macrophages, neutrophils, collagen fibers, elastic fibers, MMP-9- and MMP-12-positive cells, and iNOS- and 8-iso-PGF2-positive cells. compared to SAL and SALBC groups. compared to ELA group.

Alveolar septum
Inflammatory response	SAL	ELA	SALBC	ELABC
Macrophages (cells/10^4 μm^2)	3.55 ± 0.3		5.00 ± 0.3
Neutrophils (cells/10^4 μm^2)	5.87 ± 0.6		9.33 ± 0.7
TNFα (cells/10^4 μm^2)	2.21 ± 0.2		2.43 ± 0.3
Remodelling response	SAL	ELA	SALBC	ELABC
Collagen fibers (%)	8.80 ± 0.1		8.96 ± 0.1
Elastic fibers (%)	0.32 ± 0.03		0.37 ± 0.02
MMP-9-positive cells/10^4 μm^2	8.63 ± 1.2		9.89 ± 0.7
MMP-12-positive cells/10^4 μm^2	10.88 ± 1.4		11.22 ± 0.5
TIMP-1-positive cells/10^4 μm^2	3.55 ± 0.3		5.00 ± 0.3
Oxidative stress response	SAL	ELA	SALBC	ELABC
iNOS-positive cells/10^4 μm^2	5.51 ± 0.2		6.03 ± 0.5
eNOS-positive cells/10^4 μm^2	3.06 ± 0.2		3.58 ± 0.2	8.13 ± 0.4
8-iso-PGF2α-positive cells/10^4 μm^2	6.17 ± 0.6		6,74 ± 0.3