Serum miRNAs miR-23a, 206, and 499 as Potential Biomarkers for Skeletal Muscle Atrophy

Figure 1

Change in muscle weight and cross section areas following HU. (a–c) The hindlimb skeletal muscles (soleus, gastrocnemius, and plantaris) were weighed during 14 days (0, 3, 7, and 14 days) of hindlimb unloading. Data were showed with mean ± SEM ( for each group, versus Con). (d) The relative weight of hindlimb skeletal muscles (gastrocnemius, soleus, and plantaris) was shown during 14 days (0, 3, 7, and 14 days) of hindlimb unloading. The value of muscle/body weight ratio of Con was taken as 1 in each determination. Data were showed with mean ± SEM ( for each group, , , and versus its own Con). (e) Representative images of immunofluorescence staining of soleus muscles in Con and hindlimb unloading (3, 7, and 14 days) group using anti-laminin (red) antibody. Scale bar: 100 um ( for each group). (f) Cross section areas of soleus muscles in Con and HU (3, 7, 14 days) group. Data were showed with mean ± SEM ( for each group, total fibers in each muscle cross section were measured, versus Con). Con, control; HU3, hindlimb unloading for 3 days; HU7, hindlimb unloading for 7 days; HU14, hindlimb unloading for 14 days.

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