The Prevalence and Replication Capacity of a Tibetan Dominant HBV Strain, C/D Recombinant

Figure 1

Gel bands during the construction procedures of HBV replicative plasmid of C/D recombinant. (a) Amplification of HBV full genome with templates extracted from serum samples. Lines 1–3 were amplification products. Line 4 was λDNA/EcoT14 digested marker. (b) Amplification of HBV fragment of 2.4 Kb with amplified and ligated HBV genomic DNA as templates. Lines 1–3 were amplification products. Line 4 was λDNA/EcoT14 digested marker. (c) Amplification of HBV fragment of 1.7 Kb with amplified and ligated HBV genomic DNA as templates. Lines 1–3 were amplification products. Line 4 was λDNA/EcoT14 digested marker. (d) Amplification of HBV full genome with newly constructed replicative plasmid. The plasmid was derived from ligation of 1.7 Kb and 2.4 Kb fragments and vector pUC18. After transfection and selection, positive DH5α clones were acquired for plasmid extraction and full viral genome amplification was conducted for verification. Lines 1 and 2 were amplification products with selected plasmid as template. Lines 3 and 4 were amplification products with only vector pUC18 as templates. Line 5 was λDNA/EcoT14 digested marker.

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