The Prevalence and Replication Capacity of a Tibetan Dominant HBV Strain, C/D Recombinant

Figure 4

HBV replication intermediates in mouse models established with pHBV4.1(D) and pHBV(C/D). Mice were injected hydrodynamically with 10 μg pHBV4.1(D) orpHBV4.1(C/D) and mouse livers were harvested 3 days after modeling. HBV replication intermediates in the liver were detected through DNA (Southern) filter hybridization (a). The image of DNA (Southern) filter hybridization was imported into Quantity One (Bio-Rad Laboratories, Life Science) and analyzed. The module of Volume Rect Tool was applied to generate rectangles covering all the bands in each lane and density of delineated bands was qualified and compared (b). The two plasmids possessed similar replication capacity in vivo.

(a)

(b)