SHISA3 Promoter Methylation Is a Potential Diagnostic and Prognostic Biomarker for Laryngeal Squamous Cell Carcinoma

Figure 1

Agarose gel image and sequencing results of SHISA3. (a) Representative methylation-specific polymerase chain reaction (MSP) results regarding SHISA3 in two paired LSCC and normal tissues. Water was used as a negative control. The methylated CpG sites were highlighted by arrow. T: tumor; N: adjacent normal tissue; M: methylation; U: unmethylation. (b) The sequencing results of methylation and unmethylation PCR products.

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