SHISA3 Promoter Methylation Is a Potential Diagnostic and Prognostic Biomarker for Laryngeal Squamous Cell Carcinoma

Table 1

List of all primers used for MSP and qRT-PCR assay.

Primer	Sequence (5′ to 3′)	Amplification size (bp)
Methylation		204
Forward	AGAGGTGATCGGTAATTTTTTAGTC
Reverse	CCTATTACACAAACTCAAACTCGTT
Unmethylation		203
Forward	GAGGTGATTGGTAATTTTTTAGTTG
Reverse	CCTATTACACAAACTCAAACTCATT
SHISA3 qRT-PCR		85
Forward	GTCTACGTCCCCTTTCTCATCG
Reverse	AGGTGCAACAATAAATAGCCACT
GAPDH qRT-PCR
Forward	CCATGGAGAAGGCTGGGG	194
Reverse	CAAAGTTGTCATGGATGACC