Anticancer Effect of a Novel Octahydropyrazino[2,1-a:5,4-a′]diisoquinoline Derivative and Its Synergistic Action with Nigella sativa in Human Gastric Cancer Cells

Figure 9

Flow cytometric analysis of cultured AGS gastric adenocarcinoma cells after incubation with Nigella sativa seed oil (2.4 mg/mL), Nigella sativa seed extract (0.5 mg/mL), OM-90 (20 μM), etoposide (20 μM), Nigella sativa seed oil + OM-90 (2.4 mg/mL + 20 μM), Nigella sativa seed oil + etoposide (2.4 mg/mL + 20 μM), Nigella sativa seed methanolic extract + OM-90 (0.5 mg/mL + 30 μM), and Nigella sativa seed methanolic extract + etoposide (0.5 mg/mL + 20 μM) for 24 hours and subsequent staining with Annexin V and propidium iodide (PI). Dots with Annexin V−/PI− (Q3), Annexin V+/PI− (Q4), and Annexin V+/PI+ (Q2) features represent intact, early apoptotic, and necrotic cells, respectively. Mean percentage values from three independent experiments done in duplicate are presented.