Heterologous Secretory Expression and Characterization of Dimerized Bone Morphogenetic Protein 2 in Bacillus subtilis

Figure 5

Characterization of rhBMP2 monomer and dimer. (a) Western blot analysis of rhBMP2 monomer and dimer. Lane M, protein marker; Lane 1, rhBMP2 monomer showing band at ~13 kDa; Lane 2, rhBMP2 dimer showing band at ~25 kDa. (b) Native PAGE analysis of rhBMP2 monomer showing dimerized form. Lane M, protein marker; Lane 1, nonreducing rhBMP2 monomer showing band at ~26 kDa. The band is slightly above the 26 kDa marker band due to nonreducing nature of the sample. (c) 18% SDS-PAGE showing band of rhBMP2 dimer at ~25 kDa after collecting supernatant from 50 kDa Amicon Ultra centrifugal column.

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