Heterologous Secretory Expression and Characterization of Dimerized Bone Morphogenetic Protein 2 in Bacillus subtilis

Table 3

Summary of percentage recovery of rhBMP2 monomer and dimer at different steps of production from B. subtilis.

Purification steps	rhBMP (monomer)				rhBMP (dimer)
	Volume (ml)	Total protein (mg)	Purity (%)	Recovery (%)	Volume (ml)	Total protein (mg)	Purity (%)	Recovery (%)
Cultural supernatant	200	90	35	100	200	60	25	100
Concentrated by freeze drying	10	89	35	98	5	58	25	96
FPLC	12	1	90	1.12	7	1.84	80	3.17

concentration was determined by absorbance measurements at OD₂₈₀.   % age purity was determined by densitometric analysis of 15% SDS-gel.