Cell Signaling Pathway in 12-O-Tetradecanoylphorbol-13-acetate-Induced LCN2 Gene Transcription in Esophageal Squamous Cell Carcinoma

Figure 2

Effect of MISP, KLF10, KLF15, PPP1R18, and RXRβ overexpression on the promoter activity and mRNA level of LCN2 gene. (a) Transient cotransfection analysis of LCN2 gene promoter activity. pB152 and pcDNA3 recombinants containing the five genes were cotransfected into EC109 cells, respectively. Luciferase activities of pB152 were normalized to Renilla luciferase activity and then shown relative to that of pcDNA3 control, which was set as 1. Each value represents the mean ± SD (standard deviation). The data were representative of at least two independent experiments. Transfections were carried out in triplicate for each experiment. (b) Relative luciferase activity analysis of pB152 in EC109 cells with stable expression of MISP, KLF10, KLF15, PPP1R18, and RXRβ. (c) Analyses of LCN2 mRNA level in cells with stable expression of MISP, KLF10, KLF15, PPP1R18, and RXRβ by real-time PCR. β-Actin was applied as an internal control. , .

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