Downregulation of Profilin-1 Expression Attenuates Cardiomyocytes Hypertrophy and Apoptosis Induced by Advanced Glycation End Products in H9c2 Cells

Figure 2

Effect of different inhibitors and PFN-1 Adv-shRNA adenovirus on AGEs-induced H9c2 cells hypertrophy and apoptosis. (a) Effect of different inhibitors and PFN-1 Adv-shRNA on AGEs-induced (200 μg/ml, 24 hours) ANF and β-MHC mRNA expression. (b) Representative images show different inhibitors and PFN-1 Adv-shRNA adenovirus on cell size induced by AGEs (200 μg/ml, 24 hours) (magnification: 40x; scale bar: 100 μm). (c) Representative images show different inhibitors and PFN-1 Adv-shRNA adenovirus on apoptosis induced by AGEs (200 μg/ml, 24 hours) (magnification: 20x; scale bar: 200 μm). (d) Effect of different inhibitors and PFN-1 Adv-shRNA on cleaved caspase-3 protein expression induced by AGEs. Data were expressed as mean ± SD, . versus control; versus AGEs; versus Adv-Control; versus A + Adv-Control.

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