Retinoblastoma Binding Protein 5 Correlates with the Progression in Hepatocellular Carcinoma

Figure 3

Knockdown of RBBP5 inhibits cell cycle progress and proliferation of HCC cells. (a) Western blot analysis showed that siRBBP5#2 displayed the most significant knockdown effect on RBBP5 expression and were selected for further experiment. After knockdown of RBBP5 by siRBBP5#2, Cyclin E and PCNA were significantly downregulated. (b) Depletion of RBBP5 inhibited cell cycle progression of SMCC-7721 and Huh7 cells. (c) CCK-8 assay showed that knockdown of RBBP5 inhibited proliferation progress of SMCC-7721 and Huh7 cells. All experiments were performed in triplicate. P < 0.05.

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