Selected Enzyme Inhibitory Effects of Euphorbia characias Extracts

Table 2

Identification of polyphenolic compounds in E. characias extracts using HPLC-ESI-FT-MSMS in negative ion mode and quantification by LC-DAD.

	Putative identification	RT (min)	g/L (mean ± SD)		MW	[M-H]^−	Molecular formula	MSMS	References
			Flowers	Leaves
1	Unknown	17.31	NQ	ND	-	353.0870		191.05
2	Unknown	18.43	NQ	ND	-	799.0616		781.04; 479.04; 300.99
3	Unknown	19.58	NQ	NQ	-	951.0724		933.06
4	Unknown	25.05	NQ	ND	-	951.0732		933.06; 613.04
5	Quercetin-glucoside	26.77		ND	464.0954	463.0873		301.07	[22]
6	Quercetin-3-O-	27.12			464.0954	463.0873		301.07	[21]
7	Ellagic	27.52		ND	302.0120	301.0200		257.10	[27]
8	Quercetin-3-O-	28.70			434.0849	433.0771		301.25	[21]
9	Quercetin-3-O-	29.52			434.0849	433.0771		353.10; 191.02	[21]
10	Quercetin-3-O-	30.49			448.1005	447.0924		329.02	[21]
11	di-O-Caffeoylquinic	30.67			516.0962	515.0800		329.02	[22]
12	Kaempferol-3-O-	33.16		ND	418.0900	417.0815		285.04	[27]
13	Kaempferol-3-O-	34.39		ND	431.0973	431.0866		285.03	[27]
14	Quercetin-3-(2-O-acetyl)-	35.00			476.0954	475.0877		301.026	[21]
15	Acacetin	39.14			460.1005	459.0980		283.03	[22]
16		41.06			302.0236	301.0347		178.99; 151.00	[21]

“”: quantified using corresponding authentic standard; “a”: quantified as equivalent of quercetin-3-O-glucoside; “b”: quantified as equivalent of chlorogenic acid; “c”: quantified as equivalent of kaempferol-3-O-glucoside; “d”: quantified as equivalent of acacetin; ND: not detected (<LOD); NQ: detected but not quantified.