Heteroplasmy Detection of Mitochondrial DNA A3243G Mutation Using Quantitative Real-Time PCR Assay Based on TaqMan-MGB Probes

Figure 1

Standard curves and real-time fluorescence PCR amplification curves. (a-b) Standard curves of wild-type (VIC, a) and mutant (FAM, b) probes. The standard samples (heteroplasmy level, 50%) were successively diluted five-fold to eight points. All samples were performed in triplicate. (c-d) Amplification curves of 100% WTP (c) and 100% MutP (d) templates. All samples were performed thirty times. (e) Amplification curves of low and high mutation rate templates under exposure to visible light with 30, 45, and 60 minutes. All samples were performed in triplicate. (f) Amplification curves of mtDNA templates with the concentration of 10 fg to 10 ng.

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