Heteroplasmy Detection of Mitochondrial DNA A3243G Mutation Using Quantitative Real-Time PCR Assay Based on TaqMan-MGB Probes

Figure 2

Regression analysis of the theoretical and measured heteroplasmy values of the standard samples with different mutation rates. (a–d) Regression analysis of the two datasets derived from the standard samples with the mutation rates of 5 to 90% (a), 0.1 to 10% (b), 4 to 10% (c), and 4.5 to 10% (d). All samples were performed in triplicate.

(a)

(b)

(c)

(d)