Heteroplasmy Detection of Mitochondrial DNA A3243G Mutation Using Quantitative Real-Time PCR Assay Based on TaqMan-MGB Probes

Figure 3

Heteroplasmy levels of mt.3243A>G mutation by TaqMan-MGB qPCR assay from positive mtDNA and nonisolated DNA samples. (a–c) Heteroplasmy levels of positives from mtDNAs (a, b), urinary sediments (a), leukocytes (a, b), mitochondria (c), or supernatants and precipitations related to isolated mitochondria (c). All samples were performed in triplicate. The data of each identical sample are expressed as means ± S.D., which are derived from triplicate determinations. <0.05; <0.01.

(a)

(b)

(c)