Heteroplasmy Detection of Mitochondrial DNA A3243G Mutation Using Quantitative Real-Time PCR Assay Based on TaqMan-MGB Probes

Table 1

Sequence of primers and probes used in the current study.

Primer/probe	Sequence (5′-3′)	mtDNA region
Forward RFLP primer	CTCCCTGTACGAAAGGACAAGAGAA	3117 to 3141
Reverse RFLP primer	TATGGGGAGGGGGGTTCATAGTA	3557 to 3579
Forward qPCR primer	ATTATACCCACACCCACCCAAGAAC	3198 to 3222
Reverse qPCR primer	ATGGGTACAATGAGGAGTAGGAGGT	3320 to 3344
Wild-type probe	VIC-CCGGGCTCTGCCAT-MGB	3236 to 3249
Mutant probe	6-FAM-CCGGGCCCTGCCAT-MGB	3236 to 3249