Heteroplasmy Detection of Mitochondrial DNA A3243G Mutation Using Quantitative Real-Time PCR Assay Based on TaqMan-MGB Probes

<table class="fixed-width table-group" id="tab2"><tr><td><table class="table"><colgroup><col style="width:4.55em"/><col style="width:6.31em"/><col style="width:7.17em"/><col style="width:9.29em"/></colgroup><tr><td class="thead-hr" colspan="4"><hr/></td></tr><tr class="thead"><td class="align_left">Samples</td><td class="align_center">VIC (Ct)</td><td class="align_center">FAM (Ct)</td><td class="align_center">Heteroplasmy levels</td></tr><tr><td class="thead-hr" colspan="4"><hr/></td></tr><tr><td class="align_left">1</td><td class="align_center">5.644±0.046</td><td class="align_center">Undetermined</td><td class="align_center">0%</td></tr><tr><td class="align_left">2</td><td class="align_center">5.433±0.046</td><td class="align_center">Undetermined</td><td class="align_center">0%</td></tr><tr><td class="align_left">3</td><td class="align_center">6.024±0.087</td><td class="align_center">Undetermined</td><td class="align_center">0%</td></tr><tr><td class="align_left">4</td><td class="align_center">5.774±0.070</td><td class="align_center">Undetermined</td><td class="align_center">0%</td></tr><tr><td class="align_left">5</td><td class="align_center">7.808±0.130</td><td class="align_center">Undetermined</td><td class="align_center">0%</td></tr><tr class="table-tr"><td colspan="4"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>Heteroplasmy levels of mt.3243A&gt;G mutation of known negative samples.</div>

BioMed Research International

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Table 2

Table 2: Heteroplasmy Detection of Mitochondrial DNA A3243G Mutation Using Quantitative Real-Time PCR Assay Based on TaqMan-MGB Probes 