Changes of Nicotinamide Phosphoribosyltransferase Expressions in Thyroid Glands of Patients with Different Thyroid Pathologies

Figure 2

Comparison of log NAMPT expressions between patients with Graves’ disease without orbitopathy (GD) and with orbitopathy (GO), toxic nodular goiters (TNG), thyroid cancers (TC), and healthy thyroid tissue samples (controls). The relative quantification with external standard was performed. The NAMPT concentration was expressed in relation to concentration of reference/normalization HPRT gene. In both cases (NAMPT and HPRT), standard curves were used to obtain the relative concentration of the target and the reference gene as well as to determine the fit coefficients of the PCR relative standard curve. Results expressed as the target/reference ratio of each sample with the efficiency correction derived from standard curves were logarithmically transformed toward normality.