Research Article
Reference Gene Selection for Quantitative Real-Time PCR of Mycelia from Lentinula edodes under High-Temperature Stress
Figure 1
Analysis of 10 reference genes using geNorm. Low M values indicate high expressional stability. In iterative steps, genes with the lowest stability (i.e., the highest M value) are removed. When the threshold value of M is smaller than 1.5, the candidate reference parameters are applicable. The two less stable genes (UBC and GTP) are eliminated.