Reference Gene Selection for Quantitative Real-Time PCR of Mycelia from Lentinula edodes under High-Temperature Stress

Table 1

Primer sequences of candidate reference genes.

Symbol	Gene name	Annotation	Primer sequence (forward/reverse)	Size (bp)
18S	18S ribosomal RNA	LE01Gene00881.t1	GCAACATCAAAACCTGGCGT; GACATTCGACAACGCCCAAC	164
EF1	Elongation factor	LE01Gene03252.t2	CGGTGTTGTCAAGGGAAAGA; GGTGACGATCATTCCAGCTT	178
ACT	Actin	LE01Gene01050.t1	GTGTTACCCATACCGTTCCC; ATCGGTCAAATCACGACCAG	89
GAPDH	Glyceraldehyde-3-phosphate; dehydrogenase	LE01Gene07114.t1	GTTCCTACCCTCGACGTTTC; GATCGAAGAGTGGTTGTCCC	180
GTP	GTP-binding protein	LE01Gene00681.t1	ACCATCGTTCACACTCCCAC; GGGAAGCTCGAACTTGGGAA	267
H2A	Histones H2A	LE01Gene03840.t1	TTTGTATCCGTGTCGTGTCC; TGGGACTATCGCACATTTCC	108
TUA	Alpha-tubulin	LE01Gene01434.t1	CGACGAGATCAGGAATGGTC; TTCCGATGGTGTAGTGACCT	110
TUB	Beta-tubulin	LE01Gene08622.t3	GACATTTGCTTCCGAACCCT; CGGACATAACAAGGGACACA	79
UBI	Ubiquitin-protein ligase E3	LE01Gene00676.t1	CGTCGGGTTCTACGAGAAAG; GTTCCGTGCTTTGTTCCTTG	133
UBC	Ubiquitin-conjugating	LE01Gene00040.t1	CTTGGAAATGAGTGGTCCCC; TTGCGGGTCTCCATTAAACC	187