Reference Gene Selection for Quantitative Real-Time PCR of Mycelia from Lentinula edodes under High-Temperature Stress

Table 2

Slopes of qRT-PCR curves for reference genes.

Gene	Equation of standard curves	Slope	Regression coefficient	Amplification efficiency
GAPDH	y = -3.131x + 31.876	-3.131	R^2 = 0.999	108.654%
18S	y = -3.226x + 23.333	-3.226	R^2 = 0.993	104.142%
ACT	y = -3.277x + 21.576	-3.277	R^2 = 0.996	101.927%
UBI	y = -3.302x + 23.212	-3.302	R^2 = 0.990	100.86%
GTP	y = -3.262x + 29.435	-3.262	R^2 = 0.999	102.559%
H2A	y = -3.185x + 23.602	-3.185	R^2 = 0.996	106.054%
TUA	y = -3.287x + 17.442	-3.287	R^2 =0.997	101.483%
UBC	y = -3.198x + 21.166	-3.198	R^2 =0.996	105.454%
EF1	y = -3.244x + 17.020	-3.244	R^2 =0.998	103.367%
TUB	y = -3.274x + 28.293	-3.274	R^2 =1	102.048%