Increased Autophagy Levels Mediate Cisplatin Resistance in Cisplatin-Resistant Cells While Also Rendering Them Vulnerable to Autophagy Induction

Figure 2

Gln deprivation reduces DNA synthesis in ddp-resistant cells. (a) EdU assays of HeLa, HeLa/ddp, HGC27, and HGC27/ddp cells. The medium was exchanged for Gln-free medium the day after seeding, and EdU assays were performed 24 h later. (b) Cell cycle analysis of HeLa, HeLa/ddp, HGC27, and HGC27/ddp cells. The medium was exchanged for Gln-free medium the day after seeding, and cell cycle distribution was analyzed 24 h later. The error bars represent the s.d. of triplicate wells of a representative experiment; P<0.01.

(a)

(b)