Increased Autophagy Levels Mediate Cisplatin Resistance in Cisplatin-Resistant Cells While Also Rendering Them Vulnerable to Autophagy Induction

Figure 4

Ddp-resistant cells have a higher level of autophagy than ddp-sensitive cells, which mediates ddp resistance. (a) Aggregation of GFP-LC3 upon autophagosome formation in HeLa and HeLa/ddp cells. Quantifications of punctate areas per cell were analyzed in three different images containing more than 15 cells. (b) Western blot analysis of LC3-II protein expression in HeLa and HeLa/ddp cells; β-actin served as the loading control. (c) Relative proliferation of HeLa/ddp cells treated with increasing concentrations of ddp after autophagy inhibition by bafilomycin A1 (1 nM) for 72 h. The error bars represent the s.d. of triplicate wells of a representative experiment; P<0.01 and P<0.001.

(a)

(b)

(c)