MicroRNA-9 Enhanced Cisplatin Sensitivity in Nonsmall Cell Lung Cancer Cells by Regulating Eukaryotic Translation Initiation Factor 5A2

Figure 3

EIF5A2 is a direct target of miR-9 in NSCLC cells. (a) TargetScan prediction of a miR-9 binding site in the EIF5A2 3′UTR. (b) RT-qPCR analysis shows that miR-9 expression was the highest in the A549 cells. (c) RT-qPCR measurement of eIF5A2 expression. (d) RT-qPCR detection of eIF5A2 levels following transfection with miR-9 mimic or inhibitor. < 0.05, < 0.01, and < 0.001 vs. control. (e) Western blot shows increased eIF5A2 levels following transfection with miR-9 mimic and decreased eIF5A2 levels following transfection with miR-9 inhibitor as compared with negative siRNA.

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