Hydrogen Sulfide Protects against Chemical Hypoxia-Induced Injury via Attenuation of ROS-Mediated Ca²⁺ Overload and Mitochondrial Dysfunction in Human Bronchial Epithelial Cells

Figure 3

Hydrogen sulfide inhibited ROS-induced intracellular [Ca²⁺ in 16HBE14o- cells. Logarithmic growth cells were incubated with calcium ion fluorescence probe Fura-2/AM and the F340/380 ratio value was detected by laser scanning confocal microscope. (a) The effect of CoCl₂ on intracellular [Ca²⁺ in different doses (0, 50, 100, 200, 400, or 1000 μmol/L). (b) NAC (1 mM, scavenger of ROS) inhibited the increase in F340/380 ratio induced by hypoxia (CoCl₂, 400 μmol/L). (c) Effects of hydrogen sulfide on [Ca²⁺ in different doses (0, 50, 100, 200, 300, 600, or 1000 μmol/L). (d) Effects of hydrogen sulfide (NaHS, 300 μmol/L) on [Ca²⁺ induced by hypoxia (CoCl₂, 400 μmol/L). The results were from 3 independent experiments (NS, p < 0.05, p < 0.01,p < 0.001 versus control group, #p < 0.05 versus hypoxia group, n=3).

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