lncRNA-MEG3 Suppresses the Proliferation and Invasion of Melanoma by Regulating CYLD Expression Mediated by Sponging miR-499-5p

Figure 8

MEG3 modulated the expression of CYLD by sponging miR-499-5p in A375 cells. (a, b) Reduced expression of MEG3 increased the miR-499-5p level; oppositely, overexpression of MEG3 decreased the miR-499-5p level. (c) Upregulation of miR-499-5p suppressed the expression of CYLD in A375 cells, whereas the cotransfection of miR-499-5p mimics with pcDNA-MEG3 attenuated the inhibitory function of miR-499-5p on the expression of CYLD in A375 cells. (d) Upregulation of MEG3 promoted the expression of CYLD, but the enhancing function of pcDNA-MEG3 was limited by the cotransfection of miR-499-5p mimics with pcDNA-MEG3 in A375 cells. (e) Downregulation of MEG3 markedly suppressed the expression of CYLD, but miR-499-5p inhibitors alleviated the impeding function of sh-MEG3 on the expression of CYLD in A375 cells. ( compared with NC group and sh-control group. compared with sh-MEG3 group. compared with NC group and pcDNA-control group. compared with pcDNA-MEG3 group. compared with NC group and miR-control group. compared with miR-499-5p mimics group.)

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