-Hederin Arrests Cell Cycle at G2/M Checkpoint and Promotes Mitochondrial Apoptosis by Blocking Nuclear Factor-B Signaling in Colon Cancer Cells

Figure 5

Blockade of NF-κB signaling is required for α-hederin induction of mitochondrial apoptosis in IL-6-stimulated SW620 cells. SW620 cells were treated with vehicle, IL-6, and/or α-hederin or PDTC at indicated concentrations for 24 h. (a) Hoechst 33258 fluorescence staining. Morphologic changes of apoptotic cells were visualized under a fluorescence microscope (200 x magnification). (b) Western blot analysis of protein abundance of cleaved-caspase-9, cleaved-caspase-3, and cleaved-PARP with quantification. Significance: P<0.01 versus control, ^#P<0.05 versus IL-6, ^##P<0.01 versus IL-6.

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